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【简答题】
完形填空. I was sitting in the surgical waiting area at the hospital waiting for word of my wife's operation. The small room was filled with other families and friends who were __1__ waiting to hear how their loved ones were doing. There was a young boy sitting quietly in the __2__ .He held a small box of __3__ in one hand and a tablet( 写字板)in the other. Every so often he'd place the tablet on his lap and draw something. As the hours passed I began to see the room almost_ _4_ _.And there was a strange silence. 'Daddy,when is Mommy going home?'the young boy asked,breaking the_ _5_ _. 'Tomorrow,'his father replied. The boy picked up his crayons and_ _6_ _something on the tablet. I _ _7 _him as he moved his feet back and forth,looking up at the ceiling and then toward the sunshine-filled window.'When's_ _8_ _,Daddy?'he asked. His father__9_ _ him over to sit by him. 'Come, ___10_ _here next to me,'he said with a gentle tone in his voice. __11__his things,the little boy walked slowly to his father's side from the corner. The man_12 _ _out and held his son by his shoulders. 'There are three days in life. Yesterday,today and tomorrow. One is over and done,one we live in,the other we__13__.We can't live in yesterday,but if we are happy enough today, we will be even happier tomorrow,'he said in an attempt to__14__the boy. The little boy looked at him for a moment and said,'If Mommy is going home tomorrow, I'll be__15__. So,I want it to be tomorrow already, Daddy.' Now hanging on every word of this__16__,I leaned forward to hear his response. It was __17 __ . 'Son,the one great thing about today is once it_ _18_ _,it is already almost tomorrow.' I thought about how_ _19_ _it was. Even in my adult life. I still live waiting for what tomorrow may_ _20_ _.Now I know that it is already 'almost tomorrow.' (     )1.A.eagerly (     )2.A.chair (     )3.A.gifts (     )4.A.dark (     )5.A.promise (     )6.A.wrote (     )7.A.followed (     )8.A.today (     )9.A.knocked (     )10A.stand (     )11.A.Gathering (     )12.A.got (     )13.A.spend (     )14.A.frighten (     )15.A.happier (     )16.A.story (     )17.A.humorous (     )18.A.ends (     )19.A.meaningful (     )20.A.happen B.patiently B.corner B.toys B.full B.rule B.drew B.found B.yesterday B.ordered B.sit B.Forgetting B.took B.waste B.satisfy B.calmer B.argument B.silly B.starts B.important B.come C.happily C.distance C.chalks C.empty C.record C.printed C.watched C.future C.called C.lie C.Saving C.set C.kill C.persuade C.smarter C.conversation C.positive C.passes C.interesting C.bring D.curiously D.shadow D.crayons D.clean D.silence D.carved D.caught D.tomorrow D.pulled D.sleep D.Choosing D.reached D.expect D.warn D.sadder D.discussion D.perfect D.lasts D.fantastic D.need
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【单选题】可以识别DNA分子中特异序列并将其切割的酶称为
A.
DNA酶(DNase)
B.
限制性核酸内切酶
C.
限制性核酸外切酶
D.
非限制性核酸内切酶
E.
非限制性核酸外切酶
【简答题】限制性内切酶特异识别和切割DNA分子中的回文结构,形成的末端有
【单选题】限制性核酸内切酶在切割DNA分子时
A.
能识别DNA分子中特异核苷酸序列
B.
随机切割DNA分子
C.
切割自身DNA分子
D.
识别DNA的冈崎片段
E.
把DNA分子水解为单核苷酸
【多选题】关于DNA重组的基本工具——限制性内切酶和DNA连接酶的说法正确的是【 】
A.
1970年美国约翰·霍普金斯大学史密斯分离出一种专一性很强的DNA限制性内切酶,能够专一识别DNA序列,并在DNA链上确定的位点处将其切开
B.
限制性内切酶具有特异性识别和切割功能,可以在任何DNA分子上特异的位点处将其剪断,获取特定DNA片断。限制性内切酶被形象地称为 “分子剪刀”
C.
1967年科学家阿尔伯、史密斯等从大肠杆菌中分离并提取出连接酶。能将DNA分子相邻两端或是被“剪刀”断开的DNA片断重新连接起来,修复好DNA链断裂口。连接酶又被形象地称为“分子针线”
D.
正是因为限制性内切酶、连接酶等一系列基因工程的“重要工具”发现,人们才能够将所需要的目标基因与基因载体重组,并顺利地导入到受体细胞中,黏在受体细胞DNA特定位置上,使基因工程得以实现
【简答题】限制性内切酶特异识别和切割DNA分子中的回文结构,形成的末端有黏性末端和_________末端。
【单选题】可识别DNA分子中的特异序列,并切割DNA的酶称
A.
限制性核酸外切酶
B.
限制性核酸内切酶
C.
非限制性核酸外切酶
D.
非限制性核酸内切酶
E.
DNA酶(DNase)
【判断题】天气形势是指大范围的气压分布和大气运动状况,一般来说,在高压控制区,由于风速较大,天气多为不稳定状态,有利于污染物的稀释扩散。
A.
正确
B.
错误
【简答题】我国银铜矿储量非常丰富,其中的铜通常采用硫酸浸出提取,但浸铜时银并没有浸出而留在浸铜渣中。浸铜渣中的银含量很高,200~700g/t不等,值得回收。表7-6所示为浸铜渣的化学组成。如何从浸渣铜中提取银?
【单选题】我国政治制度的最高原则是
A.
一切权力属于人民
B.
民主集中制
C.
中国共产党的领导
D.
多党合作和政治协商
【简答题】限制性内切酶特异性识别和切割DNA分子中的回文结构形成的末端有( )和( )。
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