皮皮学，免费搜题
登录
搜题
【判断题】
在我国严重的网络犯罪行为也不需要接受刑法的相关处罚。A.
正确
B.
错误
手机使用
分享
复制链接
新浪微博
分享QQ
微信扫一扫
反馈参考答案：
举一反三
【单选题】I wanted to have him ______ the job, but she had it _____ already.
A.
do, done
B.
does, doing
C.
to do, doing
D.
to do, done
【单选题】I wanted to have him _____ the job, but he had it _____ instead.
A.
do; done
B.
does; be done
C.
to do; doing
D.
to do; done
【简答题】重组载体疫苗(recombinant vector vaccine)(名词解释)
【简答题】微机控制点火系中最佳点火提前角由 、 、 组成。
【单选题】I wanted to have him _____ the job, but she had it _____ instead.
A.
do/done
B.
does/doing
C.
to do/doing
【多选题】How is recombinant DNA cloned in bacteria?
A.
To clone a particular fragment of DNA, it is first inserted into a plasmid vector. To insert a piece of DNA into a plasmid vector, the purified plasmid DNA is opened up by a restriction enzyme that cleaves it at a single site, and the DNA fragment to be cloned is then spliced into that site using DNA ligase. This recombinant DNA molecule is now ready to be introduced into a bacterium, where it will be copied and amplified.
B.
The resulting recombinant plasmid DNA is then introduced into a bacterium by transformation. These bacteria are then suspended in a nutrient-rich broth and allowed to proliferate.
C.
In 24 hours, the engineered cells will produce hundreds of millions of copies of the plasmid, along with the DNA fragment it contains. The bacteria can then be split open (lysed) and the plasmid DNA purified from the rest of the cell contents, including the large bacterial chromosome.
D.
The DNA fragment can be readily recovered by cutting it out of the plasmid DNA with the same restriction enzyme that was used to insert it, and then separating it from the plasmid DNA by gel electrophoresis. Together, these steps allow the amplification and purification of any segment of DNA from the genome of any organism.
相关题目：
参考解析：
知识点：
题目纠错 0
发布
