What are the assumptions of Beer’s law? 2. (1) Calculate the molar absorptivity of an analyte if a 3.4×10 -4 mol/L solution of this chemical is placed in a sample cell with a path length of 5.0 cm and is found to give a value for %T of 67.4 at 450 nm;(2) Do you have any other better method to determine molar absorptivity? 3 . The following results were obtained by a clinical chemist when measuring morphine at 285 nm and using a 1.00 cm square cuvette as the sample holder. Morphine concentration (mol/L) Absorbance 0.00005 0.229 0.0001 0.308 0.0002 0.467 0.0005 0.942 (1) Why do you think the clinical choose 285 nm as the measuring wavelength? (2) Why square cuvette is used here as the sample holder? What material is the cuvette made of? (3) Prepare a Beer's law plot for these data. Use this plot to determine the concentration of morphine in an unknown sample that gives a measured absorbance of 0.615 in this assay. 4. Go through UV-Vis spectroscopy in chapter 18. Pay more attention on what are the possible energy transitions of organic and inorganic compounds? What are the components and the respective functions of UV-Vis spectrometer?